GFP anticorps (full length)

N° du produit ABIN2464056

Cet anticorps anti-GFP est un anticorps Souris Monoclonal détectant GFP dans WB et ELISA. Adapté pour Aequorea victoria.

Aperçu rapide pour GFP anticorps (full length) (ABIN2464056)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 4B10B2

Détail du produit anti-GFP anticorps

Épitope: full length

Immunogène: Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Stock: -20 °C

Stockage commentaire: GFP monoclonal antibody can be stored at -20 °C, stable for one year.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

ID gène: 7011691

NCBI Accession: YP_002302326

UniProt: B6UPG7